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排序方式: 共有111条查询结果,搜索用时 31 毫秒
71.
Pollination–seed predation mutualisms such as moth–yucca interactions are important model systems for studying mechanisms that limit exploitation when mutualistic partners have strong conflicts of interest. In many moth–yucca interactions, oviposition leads to the failure of some ovules to develop normally. Here, we demonstrate that moth eggs almost always perish if they are inside these oviposition‐induced ‘damage zones’ of developing fruit. Moreover, because more ovipositions result both in larger damage zones and in higher proportions of eggs within damage zones, this source of mortality is strongly density‐dependent. Therefore, mortality of eggs in oviposition‐induced damage zones may be an important process for limiting seed consumption and regulating moth densities in many moth–yucca mutualisms. 相似文献
72.
T Yvanka de Soysa Allison Ulrich Timo Friedrich Danielle Pite Shannon L Compton Deborah Ok Rebecca L Bernardos Gerald B Downes Shizuka Hsieh Rachael Stein M Caterina Lagdameo Katherine Halvorsen Lydia-Rose Kesich Michael JF Barresi 《BMC biology》2012,10(1):1-25
Background
During nerve growth, cytoplasmic vesicles add new membrane preferentially to the growth cone located at the distal tip of extending axons. Growth cone membrane is also retrieved locally, and asymmetric retrieval facilitates membrane remodeling during growth cone repulsion by a chemorepellent gradient. Moreover, growth inhibitory factors can stimulate bulk membrane retrieval and induce growth cone collapse. Despite these functional insights, the processes mediating local membrane remodeling during axon extension remain poorly defined.Results
To investigate the spatial and temporal dynamics of membrane retrieval in actively extending growth cones, we have used a transient labeling and optical recording method that can resolve single vesicle events. Live-cell confocal imaging revealed rapid membrane retrieval by distinct endocytic modes based on spatial distribution in Xenopus spinal neuron growth cones. These modes include endocytic "hot-spots" triggered at the base of filopodia, at the lateral margins of lamellipodia, and along dorsal ridges of the growth cone. Additionally, waves of endocytosis were induced when individual filopodia detached from the substrate and fused with the growth cone dorsal surface or with other filopodia. Vesicle formation at sites of membrane remodeling by self-contact required F-actin polymerization. Moreover, bulk membrane retrieval by macroendocytosis correlated positively with the substrate-dependent rate of axon extension and required the function of Rho-family GTPases.Conclusions
This study provides insight into the dynamic membrane remodeling processes essential for nerve growth by identifying several distinct modes of rapid membrane retrieval in the growth cone during axon extension. We found that endocytic membrane retrieval is intensified at specific subdomains and may drive the dynamic membrane ruffling and re-absorption of filopodia and lamellipodia in actively extending growth cones. The findings offer a platform for determining the molecular mechanisms of distinct endocytic processes that may remodel the surface distribution of receptors, ion channels and other membrane-associated proteins locally to drive growth cone extension and chemotactic guidance. 相似文献73.
Ashwini?S?Kucknoor Vasanthakrishna?Mundodi JF?AldereteEmail author 《BMC molecular biology》2005,6(1):5
Background
Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prominent surface protein AP65. The bovine trichomonad, Tritrichomonas foetus, adheres poorly to human VECs. Thus, we established a transfection system for heterologous expression of the T. vaginalis AP65 in T. foetus, as an alternative approach to confirm adhesin function for this virulence factor. 相似文献74.
Vincens P; Buffat L; Andre C; Chevrolat JP; Boisvieux JF; Hazout S 《Bioinformatics (Oxford, England)》1998,14(8):715-725
MOTIVATION: Complete genomic sequences will become available in the future.
New methods to deal with very large sequences (sizes beyond 100 kb)
efficiently are required. One of the main aims of such work is to increase
our understanding of genome organization and evolution. This requires
studies of the locations of regions of similarity. RESULTS: We present here
a new tool, ASSIRC ('Accelerated Search for SImilarity Regions in
Chromosomes'), for finding regions of similarity in genomic sequences. The
method involves three steps: (i) identification of short exact chains of
fixed size, called 'seeds', common to both sequences, using hashing
functions; (ii) extension of these seeds into putative regions of
similarity by a 'random walk' procedure; (iii) final selection of regions
of similarity by assessing alignments of the putative sequences. We used
simulations to estimate the proportion of regions of similarity not
detected for particular region sizes, base identity proportions and seed
sizes. This approach can be tailored to the user's specifications. We
looked for regions of similarity between two yeast chromosomes (V and IX).
The efficiency of the approach was compared to those of conventional
programs BLAST and FASTA, by assessing CPU time required and the regions of
similarity found for the same data set. AVAILABILITY: Source programs are
freely available at the following address: ftp://ftp.biologie.ens.
fr/pub/molbio/assirc.tar.gz CONTACT: vincens@biologie.ens.fr,
hazout@urbb.jussieu.fr
相似文献
75.
Auxin and gibberellin effects on cell growth and starch during abscission in cotton 总被引:2,自引:1,他引:1 下载免费PDF全文
An increase in starch content of cells in the abscission zone of the cotton explant appeared correlated with an increase in number of cells. A large increase in the number of cells in the abscission zone, concomitant with an increase in starch content, followed treatment with gibberellin as compared to auxin. In the final stages of abscission starch was hydrolyzed in the cells of the separation layer. Some starch remained after the petiole abscised.
A positive phloroglucinol-hydrochloric acid reaction in the cells of the petiole distal to the line of separation indicated the presence, not of lignin, but of soluble sugars and uronic acids. This reaction was especially intense following gibberellic acid treatment.
It was concluded that gibberellin in accelerating abscission leads to (1) an increase in cell number and starch content in the abscission zone, (2) the hydrolysis of starch in the separation layer just before abscission, and (3) the breakdown of polysaccharides and the release of soluble sugars and uronic acids. Auxin, an abscission retardant, either delays or prevents these events.
相似文献76.
JP Valde LG Lawson A Lindberg JF Agger H Saloniemi O Østerås 《Acta veterinaria Scandinavica》2004,45(4):201-210
Data from the national dairy cow recording systems during 1997 were used to calculate lactation-specific cumulative risk of mastitis treatments and cumulative risk of removal from the herds in Denmark, Finland Norway and Sweden. Sweden had the lowest risk of recorded mastitis treatments during 305 days of lactation and Norway had the highest risk. The incidence risk of recorded mastitis treatments during 305 days of lactation in Denmark, Finland, Norway and Sweden was 0.177, 0.139, 0.215 and 0.127 for first parity cows and 0.228, 0.215, 0.358 and 0.204 for parities higher than three, respectively. The risk of a first parity cow being treated for mastitis was almost 3 times higher at calving in Norway than in Sweden. The period with the highest risk for mastitis treatments was from 2 days before calving until 14 days after calving and the highest risk for removal was from calving to 10 days after calving in all countries.The study clearly demonstrated differences in bovine mastitis treatment patterns among the Nordic countries. The most important findings were the differences in treatment risks during different lactations within each country, as well as differences in strategies with respect to the time during lactation mastitis was treated. 相似文献
77.
Penelope R Whitehorn Matthew C Tinsley Mark JF Brown Ben Darvill Dave Goulson 《BMC evolutionary biology》2009,9(1):152-9
Background
Inbreeding and the loss of genetic diversity are known to be significant threats to small, isolated populations. Hymenoptera represent a special case regarding the impact of inbreeding. Haplodiploidy may permit purging of deleterious recessive alleles in haploid males, meaning inbreeding depression is reduced relative to diploid species. In contrast, the impact of inbreeding may be exacerbated in Hymenopteran species that have a single-locus complementary sex determination system, due to the production of sterile or inviable diploid males. We investigated the costs of brother-sister mating in the bumblebee Bombus terrestris. We compared inbred colonies that produced diploid males and inbred colonies that did not produce diploid males with outbred colonies. Mating, hibernation and colony founding took place in the laboratory. Once colonies had produced 15 offspring they were placed in the field and left to forage under natural conditions. 相似文献78.
Background
Trichomonas vaginalis is a human urogenital pathogen responsible for trichomonosis, the number-one, non-viral sexually transmitted disease (STD) worldwide, while T. tenax is a commensal of the human oral cavity, found particularly in patients with poor oral hygiene and advanced periodontal disease. The extent of genetic identity between T. vaginalis and its oral commensal counterpart is unknown. 相似文献79.
In pollination–seed predation mutualisms between yuccas and yucca moths, conflicts of interest exist for yuccas, because benefits of increased pollination may be outweighed by increased seed consumption. These conflicts raise the problem of what limits seed consumption, and ultimately what limits or regulates moth populations. Although the current hypothesis is that yuccas should selectively abscise flowers with high numbers of yucca-moth eggs, within-inflorescence selective abscission occurs in only one of the three moth–yucca systems that we studied. It occurs only when oviposition directly damages developing ovules, and does not, therefore, provide a general explanation for the resolution of moth–yucca conflicts. Within-locule egg mortality provides an alternative and stronger mechanism for limiting seed damage, and generating density-dependent mortality for yucca-moth populations. However, the most important feature of moth–yucca systems is that they are diverse, encompassing multiple modes of interaction, each with different consequences for limiting and regulating yucca moths. 相似文献
80.
Early origin of foraminifera suggested by SSU rRNA gene sequences 总被引:11,自引:3,他引:8
Pawlowski J; Bolivar I; Fahrni JF; Cavalier-Smith T; Gouy M 《Molecular biology and evolution》1996,13(3):445-450
Foraminifera are one of the largest groups of unicellular eukaryotes with
probably the best known fossil record. However, the origin of foraminifera
and their phylogenetic relationships with other eukaryotes are not well
established. In particular, two recent reports, based on ribosomal RNA gene
sequences, have reached strikingly different conclusions about
foraminifera's evolutionary position within eukaryotes. Here, we present
the complete small subunit (SSU) rRNA gene sequences of three species of
foraminifera. Phylogenetic analysis of these sequences indicates that they
branch very deeply in the eukaryotic evolutionary tree: later than those of
the amitochondrial Archezoa, but earlier than those of the Euglenozoa and
other mitochondria-bearing phyla. Foraminifera are clearly among the
earliest eukaryotes with mitochondria, but because of the peculiar nature
of their SSU genes we cannot be certain that they diverged first, as our
data suggest.
相似文献